Structure and co-occurrence patterns of bacterial communities associated with white faeces disease outbreaks in Pacific white-leg shrimp Penaeus vannamei aquaculture.

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Alfiansah, Yustian ORCID: https://orcid.org/0000-0002-1640-9834, Peters, Sonja, Harder, Jens, Hassenrück, Christiane and Gärdes, Astrid (2020) Structure and co-occurrence patterns of bacterial communities associated with white faeces disease outbreaks in Pacific white-leg shrimp Penaeus vannamei aquaculture. Scientific Reports, 10 (1). DOI https://doi.org/10.1038/s41598-020-68891-6.

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Abstract

Bacterial diseases cause production failures in shrimp aquacultures. To understand environmental conditions and bacterial community dynamics contributing to white faeces disease (WFD) events, we analysed water quality and compared bacterial communities in water as well as in intestines and faeces of healthy and diseased shrimps, respectively, via 16S rRNA gene sequencing and qPCR of transmembrane regulatory protein (toxR), thermolabile haemolysin (tlh), and thermostable direct haemolysin genes of pathogenic Vibrio parahaemolyticus as a proxy for virulence. WFD occurred when pH decreased to 7.71–7.84, and Alteromonas, Pseudoalteromonas and Vibrio dominated the aquatic bacterial communities. The disease severity further correlated with increased proportions of Alteromonas, Photobacterium, Pseudoalteromonas and Vibrio in shrimp faeces. These opportunistic pathogenic bacteria constituted up to 60% and 80% of the sequences in samples from the early and advances stages of the disease outbreak, respectively, and exhibited a high degree of co-occurrence. Furthermore, toxR and tlh were detected in water at the disease event only. Notably, bacterial community resilience in water occurred when pH was adjusted to 8. Then WFD ceased without a mortality event. In conclusion, pH was a reliable indicator of the WFD outbreak risk. Dissolved oxygen and compositions of water and intestinal bacteria may also serve as indicators for better prevention of WFD events.

Document Type: Article
Programme Area: UNSPECIFIED
Research affiliation: Biogeochemistry and Geology > Tropical Marine Microbiology
Social Sciences > Development and Knowledge Sociology
Refereed: Yes
Open Access Journal?: Yes
DOI: https://doi.org/10.1038/s41598-020-68891-6
ISSN: 2045-2322
Date Deposited: 06 Aug 2021 14:45
Last Modified: 26 Mar 2024 13:31
URI: http://cris.leibniz-zmt.de/id/eprint/4697

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