Effect of Oxylipins, Terpenoid Precursors and Wounding on Soft Corals’ Secondary Metabolism as Analyzed via UPLC/MS and Chemometrics.
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Farag, Mohamed, Westphal, Hildegard 
ORCID: https://orcid.org/0000-0001-7324-6122, Eissa, Tarek, Wessjohann, Ludger and Meyer, Achim ORCID: https://orcid.org/0000-0002-4345-274X
(2017)
Effect of Oxylipins, Terpenoid Precursors and Wounding on Soft Corals’ Secondary Metabolism as Analyzed via UPLC/MS and Chemometrics.
Molecules, 22
(12).
p. 2195.
DOI https://doi.org/10.3390/molecules22122195.
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Abstract
he effect of three oxylipin analogues, a terpenoid intermediate and wounding on the secondary metabolism of the soft corals Sarcophyton glaucum and Lobophyton pauciflorum was assessed. Examined oxylipins included prostaglandin (PG-E1), methyl jasmonate (MeJA), and arachidonic acid (AA) in addition to the diterpene precursor geranylgeranylpyrophosphate (GGP). Post-elicitation, metabolites were extracted from coral heads and analyzed via UPLC-MS followed by multivariate data analyses. Both supervised and unsupervised data analyses were used for sample classification. Multivariate data analysis revealed clear segregation of PG-E1 and MeJA elicited S. glaucum at 24 and 48 h post elicitation from other elicitor samples and unelicited control group. PG-E1 was found more effective in upregulating S. glaucum terpene/sterol levels compared to MeJA. Metabolites showing upregulation in S. glaucum include campestene-triol and a cembranoid, detected at ca. 30- and 2-fold higher levels compared to unelicited corals. Such an elicitation effect was less notable in the other coral species L. pauciflorum, suggesting a differential oxylipin response in soft corals. Compared to MeJA and PG, no elicitation effect was observed for GGP, AA or wounding on the metabolism of either coral species.
| Document Type: | Article |
|---|---|
| Programme Area: | UNSPECIFIED |
| Research affiliation: | Infrastructure > Biology Laboratory Biogeochemistry and Geology > Geoecology & Carbonate Sedimentology |
| Refereed: | Yes |
| Open Access Journal?: | Yes |
| DOI: | https://doi.org/10.3390/molecules22122195 |
| ISSN: | 1420-3049 |
| Date Deposited: | 05 Jun 2019 09:57 |
| Last Modified: | 01 Oct 2020 12:58 |
| URI: | http://cris.leibniz-zmt.de/id/eprint/1996 |
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